Alpha Medical Lab

25-Hydroxyvitamin D (D2, D3), LC/MS/MS

Ordered Test Code:

D2D3VITD

Lab Test Component	Requirement
Test Name	25-Hydroxyvitamin D (D2, D3), LC/MS/MS
Alpha’s Order Test Code	D2D3VITD
Specimen Collection Tube	Red Top/0.5mL
Collection Tube Handling
Minimum Volume	0.3mL
Transport Temperture	Refrigerated
Stability	Refrigerated: 21 days; Frozen: 21 days
Collection Instructions	“0.5mL serum/Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately. Alternatively, collect blood in a Serum Separator Tube (SST®), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.”

Clinical Significance

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 – the endogenous form of the vitamin and 25-OH D2 – the analog form used to treat 25-OH Vitamin D3 deficiency.